@text
"QUATERNARY  STRUCTURE  --  THE  COILED-COIL  (LEUCINE  ZIPPER)"

Quaternary structure is defined as the way in which two or more amino acid chains (polypeptides; subunits) associate to form a working (active) protein.  Some of the proteins that you have looked at to this point are fully functional as a single amino acid chain (e.g., hemerythrin & Ras).  Proteins such as these are considered 'monomeric'.  Other proteins are only functional when two or more chains associate to form multimeric structures (e.g., TIM - active as a dimer (2 chains); porin - active as a trimer (3 chains)).

The coiled-coil describes the portion of a dimeric structure where two identical helices, one contributed by each chain, wind around each other.  In the 'leucine zipper', the helices are kept together by favorable sidechain interactions primarily involving leucines.  This 'zipper' acts as a scaffold for some proteins which bind DNA in higher organisms.  In other words, the zipper keeps the protein in a dimeric form, allowing another part of the protein to bind DNA.  You will be able to see in Kinemage 1, how the leucines repeat every seventh position and interact hydrophobically with one another between chains (shown here:  GCN4; from PDB file, 2ZTA).  As in the initial helix you examined, sidechains pointing away from the zipper interface are exposed to aqueous solution and are therefore hydrophilic.  You can look at the different sidechains by clicking the 'inner' and 'outer' sidechain buttons.  View2 presents an excellent view through the zipper interface.  Be sure to examine both views with all sidechains turned off at some point; this will give you a feel for the 'coiling' of the two helices.  Kinemage 2 shows the extensive coiling of a bacterial protein called tropomyosin (from PDB file, 2TMA).
@kinemage 1
@caption
Leucine zipper from GCN4; Calpha trace shown for each backbone.  Leucines are highlighted in red; other sidechains at the interface are shown in gold.  Select view2 for a good look through the zipper interface.
@onewidth
@viewid {Side}
@zoom 0.94 
@zslab 140 
@center 19.365 15.467 4.248 
@matrix 
-0.37936 -0.85235  0.35997 -0.74156  0.04742 -0.66920  0.55333 -0.52081 -0.65006
@2viewid {Thru coil}
@2zoom 1.31 
@2zslab 290 
@2center 20.302 16.927 9.304 
@2matrix 
-0.40970  0.41326  0.81324 -0.73300 -0.67981 -0.02382  0.54301 -0.60586  0.58144
@group {"ZIPPER"}
@vectorlist {Helix 1} color= white 
{ca arg 1} P 38.127 14.684 15.695
{ca met 2} 36.66 18.155 15.84
{ca lys 3} 37.33 19.06 12.267
{ca gln 4} 35.67 15.982 10.84
{ca leu 5} 32.643 16.6 13.126
{ca glu 6} 32.395 20.303 12.225
{ca asp 7} 32.448 19.407 8.572
{ca lys 8} 29.714 16.773 9.066
{ca val 9} 27.517 19.463 10.67
{ca glu 10} 28.153 21.876 7.674
{ca glu 11} 27.355 19.15 5.237
{ca leu 12} 24.14 18.084 6.963
{ca leu 13} 22.953 21.628 7.228
{ca ser 14} 23.635 22.12 3.513
{ca lys 15} 21.673 18.905 2.595
{ca asn 16} 18.92 20.03 4.916
{ca tyr 17} 18.583 23.458 3.27
{ca his 18} 18.464 21.654 -0.143
{ca leu 19} 15.562 19.515 1.148
{ca glu 20} 13.734 22.442 2.62
{ca asn 21} 14.082 24.25 -.792
{ca glu 22} 12.464 21.235 -2.495
{ca val 23} 9.567 21.098 .055
{ca ala 24} 8.696 24.74 -.647
{ca arg 25} 8.757 24.218 -4.31
{ca leu 26} 6.58 21.183 -3.91
{ca lys 27} 4.334 23.132 -1.617
{ca lys 28} 3.872 25.752 -4.34
{ca leu 29} 2.917 22.842 -6.655
{ca val 30} .184 21.71 -4.154
{ca gly 31} -1.672 24.948 -4.007
@vectorlist {Helix 2} color=paleyellow
{ca arg 1} P 32.954 20.476 22.253
{ca met 2} 33.142 16.905 20.93
{ca lys 3} 29.997 15.7 22.554
{ca gln 4} 27.846 18.716 21.617
{ca leu 5} 28.993 18.455 17.966
{ca glu 6} 28.047 14.698 17.988
{ca asp 7} 24.653 15.646 19.293
{ca lys 8} 24.16 18.326 16.627
{ca val 9} 25.022 15.78 13.85
{ca glu 10} 22.418 13.406 15.44
{ca glu 11} 19.857 16.105 15.505
{ca leu 12} 20.537 17.187 11.822
{ca leu 13} 20.506 13.546 10.735
{ca ser 14} 16.977 13.025 12.308
{ca lys 15} 15.768 16.242 10.722
{ca asn 16} 17.148 15.287 7.314
{ca tyr 17} 15.212 11.918 7.396
{ca his 18} 12.044 13.664 8.32
{ca leu 19} 12.542 16.087 5.334
{ca glu 20} 13.32 13.222 2.974
{ca asn 21} 10.096 11.435 4.006
{ca glu 22} 8.192 14.685 3.595
{ca val 23} 9.67 15.232 .157
{ca ala 24} 8.7 11.725 -.835
{ca arg 25} 5.052 12.23 .462
{ca leu 26} 4.613 15.62 -1.37
{ca lys 27} 6.086 14.333 -4.63
{ca lys 28} 3.73 11.297 -4.703
{ca leu 29} .8 13.434 -3.964
{ca val 30} 1.817 16.156 -6.362
{ca gly 31} 3.33 14.592 -9.406
@group {Sidechains}
@subgroup {Leucines} dominant
@vectorlist {Leu} color=red
{ca leu 5} P 32.643 16.6 13.126
{cb leu 5} 32.46 16.42 14.67
{cg leu 5} 32.495 15.02 15.313
{cd1 leu 5} 32.86 15.235 16.79
{cg leu 5} P 32.495 15.02 15.313
{cd2 leu 5} 31.102 14.392 15.26
{ca leu 12} P 24.14 18.084 6.963
{cb leu 12} 23.9 17.347 8.3
{cg leu 12} 24.408 15.918 8.203
{cd1 leu 12} 24.58 15.3 9.56
{cg leu 12} P 24.408 15.918 8.203
{cd2 leu 12} 23.332 15.098 7.556
{ca leu 19} P 15.562 19.515 1.148
{cb leu 19} 15.57 18.356 2.134
{cg leu 19} 16.384 17.163 1.675
{cd1 leu 19} 16.71 16.315 2.883
{cg leu 19} P 16.384 17.163 1.675
{cd2 leu 19} 15.594 16.277 .73
{ca leu 26} P 6.58 21.183 -3.91
{cb leu 26} 6.845 19.727 -3.578
{cg leu 26} 7.913 19.102 -4.402
{cd1 leu 26} 8.753 18.277 -3.443
{cg leu 26} P 7.913 19.102 -4.402
{cd2 leu 26} 7.178 18.174 -5.34
{ca leu 5} P 28.993 18.455 17.966
{cb leu 5} 30.46 18.668 17.427
{cg leu 5} 31.045 20.115 17.486
{cd1 leu 5} 32.538 20.142 17.182
{cg leu 5} P 31.045 20.115 17.486
{cd2 leu 5} 30.427 21.023 16.45
{ca leu 12} P 20.537 17.187 11.822
{cb leu 12} 21.825 17.848 11.224
{cg leu 12} 22. 19.29 11.694
{cd1 leu 12} 23.31 19.983 11.275
{cg leu 12} P 22. 19.29 11.694
{cd2 leu 12} 20.78 20.092 11.305
{ca leu 19} P 12.542 16.087 5.334
{cb leu 19} 13.622 17.152 5.14
{cg leu 19} 13.501 18.332 6.097
{cd1 leu 19} 14.723 19.256 5.994
{cg leu 19} P 13.5 18.332 6.097
{cd2 leu 19} 12.203 19.1 5.877
{ca leu 26} P 4.613 15.62 -1.37
{cb leu 26} 5.357 16.95 -1.034
{cg leu 26} 4.935 17.73 .23
{cd1 leu 26} 5.716 19.032 .37
{cg leu 26} P 4.935 17.73 .23
{cd2 leu 26} 3.495 18.16 .132
@subgroup {Others}
@vectorlist {inner} color=gold
{ca asn 16} P 17.148 15.287 7.314
{cb asn 16} 18.628 14.868 7.155
{cg asn 16} 19.12 14.552 5.75
{od1 asn 16} 19.236 13.376 5.377
{cg asn 16} P 19.12 14.552 5.75
{nd2 asn 16} 19.58 15.576 5.036
{ca asn 16} P 18.92 20.03 4.916
{cb asn 16} 18.758 20.06 6.426
{cg asn 16} 18.773 18.704 7.105
{od1 asn 16} 18.912 17.604 6.512
{cg asn 16} P 18.773 18.704 7.105
{nd2 asn 16} 18.717 18.82 8.413
{ca met 2} P 36.66 18.155 15.84
{cb met 2} 37.212 19.245 16.74
{cg met 2} 36.06 19.824 17.54
{sd met 2} 36.542 20.556 19.116
{ce met 2} 38.18 21.188 18.664
{ca val 9} P 27.517 19.463 10.67
{cb val 9} 27.758 20.28 12.002
{cg1 val 9} 26.726 21.386 12.216
{cb val 9} P 27.758 20.28 12.002
{cg2 val 9} 27.75 19.406 13.26
{ca val 23} P 9.567 21.098 0.055
{cb val 23} 9.673 20.85 1.6
{cg1 val 23} 8.345 21.088 2.316
{cb val 23} P 9.673 20.85 1.6
{cg2 val 23} 10.167 19.442 1.954
{ca val 30} P .184 21.71 -4.154
{cb val 30} .49 21.29 -2.708
{cg1 val 30} -.783 21.014 -1.924
{cb val 30} P .49 21.29 -2.708
{cg2 val 30} 1.285 20. -2.74
{ca met 2} P 33.142 16.905 20.93
{cb met 2} 34.172 15.858 21.36
{cg met 2} 34.306 14.67 20.426
{sd met 2} 35.754 13.637 20.782
{ce met 2} 35.522 12.378 19.5
{ca val 9} P 25.022 15.78 13.85
{cb val 9} 26.336 14.944 13.536
{cg1 val 9} 26.186 13.72 12.597
{cb val 9} P 26.336 14.944 13.536
{cg2 val 9} 27.54 15.796 13.117
{ca val 23} P 9.67 15.232 .157
{cb val 23} 11.153 15.505 -.104
{cg1 val 23} 11.506 15.2 -1.554
{cb val 23} P 11.153 15.505 -.104
{cg2 val 23} 11.43 16.974 .18
{ca val 30} P 1.817 16.156 -6.362
{cb val 30} 2.757 17.322 -6.056
{cg1 val 30} 3.265 17.998 -7.32
{cb val 30} P 2.757 17.322 -6.056
{cg2 val 30} 2.024 18.36 -5.224
@vectorlist {outer} color=cyan
{ca lys 3} P 37.33 19.06 12.267
{cb lys 3} 38.693 18.97 11.607
{cg lys 3} 38.865 19.967 10.478
{cd lys 3} 40.277 19.857 9.96
{ce lys 3} 41.133 19.056 10.944
{nz lys 3} 42.277 18.355 10.336
{cd gln 4} P 36.35 13.638 8.622
{ne2 gln 4} 36.207 12.357 8.333
{cd gln 4} P 36.35 13.638 8.622
{oe1 gln 4} 36.06 14.53 7.794
{cg gln 4} P 36.973 13.932 10.002
{cd gln 4} 36.35 13.638 8.622
{cb gln 4} P 36.016 14.498 11.074
{cg gln 4} 36.973 13.932 10.002
{ca gln 4} P 35.67 15.982 10.84
{cb gln 4} 36.016 14.498 11.074
{ca asp 7} P 32.448 19.407 8.572
{cb asp 7} 33.61 18.684 7.924
{cg asp 7} 34.776 19.59 7.745
{od1 asp 7} 34.733 20.818 7.83
{cg asp 7} P 34.776 19.59 7.745
{od2 asp 7} 35.858 18.89 7.598
{ca glu 10} P 28.153 21.876 7.674
{cb glu 10} 29.542 22.47 7.407
{cg glu 10} 30.08 23.27 8.632
{cd glu 10} 31.53 23.665 8.413
{oe1 glu 10} 32.186 23.223 7.495
{cd glu 10} P 31.53 23.665 8.413
{oe2 glu 10} 32.014 24.54 9.265
{ca glu 11} P 27.355 19.15 5.237
{cb glu 11} 28.092 17.82 5.028
{cg glu 11} 27.313 16.745 4.213
{cd glu 11} 28.113 15.46 4.232
{oe1 glu 11} 28.883 15.228 5.116
{cd glu 11} P 28.113 15.46 4.232
{oe2 glu 11} 28.04 14.694 3.17
{ca ser 14} P 23.635 22.12 3.513
{cb ser 14} 25.053 21.978 3.015
{og ser 14} 25.028 22.417 1.696
{ca tyr 17} P 18.583 23.458 3.27
{cb tyr 17} 19.767 24.427 3.22
{cg tyr 17} 19.852 25.203 4.516
{cd1 tyr 17} 18.732 25.309 5.312
{ce1 tyr 17} 18.76 26.03 6.522
{cg tyr 17} P 19.852 25.203 4.516
{cd2 tyr 17} 21.034 25.85 4.94
{ce2 tyr 17} 21.07 26.603 6.098
{ce1 tyr 17} P 18.76 26.03 6.522
{cz tyr 17} 19.922 26.66 6.918
{oh tyr 17} 19.958 27.387 8.08
{ce2 tyr 17} P 21.07 26.603 6.098
{cz tyr 17} 19.922 26.66 6.918
{ca his 18} P 18.464 21.654 -.143
{cb his 18} 19.42 20.613 -.782
{cg his 18} 18.81 20.08 -2.06
{cd2 his 18} 18.57 20.72 -3.238
{cg his 18} P 18.81 20.08 -2.06
{nd1 his 18} 18.302 18.787 -2.186
{ce1 his 18} 17.794 18.68 -3.415
{ne2 his 18} 17.93 19.832 -4.065
{cd2 his 18} 18.57 20.72 -3.238
{ca asn 21} P 14.082 24.25 -.792
{cb asn 21} 15.37 24.586 -1.597
{cg asn 21} 16.196 25.65 -.906
{od1 asn 21} 15.673 26.347 -.032
{cg asn 21} P 16.196 25.65 -.906
{nd2 asn 21} 17.494 25.722 -1.216
{ca ala 24} P 8.696 24.74 -.647
{cb ala 24} 9.749 25.784 -.402
{ca arg 25} P 8.757 24.218 -4.31
{cb arg 25} 10.014 23.74 -5.01
{cg arg 25} 9.894 23.276 -6.443
{cd arg 25} 10.867 22.15 -6.77
{ne arg 25} 10.165 21.277 -7.66
{cz arg 25} 10.132 19.966 -7.72
{nh1 arg 25} 9.364 19.415 -8.63
{cz arg 25} P 10.132 19.966 -7.72
{nh2 arg 25} 10.852 19.185 -6.915
{ca lys 28} P 3.872 25.752 -4.34
{cb lys 28} 5.106 26.474 -4.846
{cg lys 28} 4.792 27.465 -5.937
{cd lys 28} 5.94 27.568 -6.93
{ce lys 28} 5.568 27.375 -8.4
{nz lys 28} 6.746 27.234 -9.285
{ca lys 3} P 29.997 15.7 22.554
{cb lys 3} 29.92 15.16 23.96
{cg lys 3} 30.7 13.862 24.217
{cd lys 3} 31.11 13.743 25.71
{ce lys 3} 30.39 12.69 26.602
{nz lys 3} 30.08 13.095 28.015
{ca gln 4} P 27.846 18.716 21.617
{cb gln 4} 28.164 20.13 22.097
{cg gln 4} 27.757 20.263 23.57
{cd gln 4} 27.464 21.684 24.02
{oe1 gln 4} 26.806 22.478 23.338
{cd gln 4} P 27.464 21.684 24.02
{ne2 gln 4} 27.942 22.014 25.207
{ca asp 7} P 24.653 15.646 19.293
{cb asp 7} 24.307 16.28 20.662
{cg asp 7} 24.64 15.433 21.863
{od1 asp 7} 24.822 14.213 21.835
{cg asp 7} P 24.64 15.433 21.863
{od2 asp 7} 24.83 16.185 22.92
{ca glu 10} P 22.418 13.406 15.44
{cb glu 10} 22.57 12.775 16.83
{cg glu 10} 23.958 12.065 16.94
{cd glu 10} 24.18 11.567 18.345
{oe1 glu 10} 23.427 11.872 19.202
{cd glu 10} P 24.18 11.567 18.345
{oe2 glu 10} 25.146 10.707 18.56
{ca glu 11} P 19.857 16.105 15.505
{cb glu 11} 19.99 17.394 16.34
{cg glu 11} 19.81 17.122 17.863
{cd glu 11} 20.296 18.194 18.846
{oe1 glu 11} 20.702 19.3 18.518
{cd glu 11} P 20.296 18.194 18.846
{oe2 glu 11} 20.218 17.813 20.114
{ca ser 14} P 16.977 13.025 12.308
{cb ser 14} 16.79 13.235 13.8
{og ser 14} 15.457 12.993 14.11
{ca tyr 17} P 15.212 11.918 7.396
{cb tyr 17} 15.426 10.776 8.407
{cg tyr 17} 16.71 10.044 8.203
{cd1 tyr 17} 17.335 10.084 6.967
{ce1 tyr 17} 18.527 9.39 6.73
{cg tyr 17} P 16.71 10.044 8.203
{cd2 tyr 17} 17.295 9.33 9.258
{ce2 tyr 17} 18.458 8.617 9.04
{ce1 tyr 17} P 18.527 9.39 6.73
{cz tyr 17} 19.063 8.652 7.77
{oh tyr 17} 20.24 8. 7.615
{ce2 tyr 17} P 18.458 8.617 9.04
{cz tyr 17} 19.063 8.652 7.77
{ca his 18} P 12.044 13.664 8.32
{cb his 18} 11.818 14.546 9.56
{cg his 18} 10.602 15.373 9.383
{cd2 his 18} 9.307 14.967 9.26
{cg his 18} P 10.602 15.373 9.383
{nd1 his 18} 10.656 16.76 9.27
{ce1 his 18} 9.4 17.154 9.072
{cd2 his 18} P 9.307 14.967 9.26
{ne2 his 18} 8.57 16.104 9.075
{ce1 his 18} P 9.4 17.154 9.072
{ne2 his 18} 8.57 16.104 9.075
{ca asn 21} P 10.096 11.435 4.006
{cb asn 21} 9.762 10.767 5.402
{cg asn 21} 10.846 9.88 6.09
{od1 asn 21} 11.946 9.57 5.582
{cg asn 21} P 10.846 9.88 6.09
{nd2 asn 21} 10.538 9.478 7.314
{ca ala 24} P 8.7 11.725 -.835
{cb ala 24} 9.236 10.518 -.105
{ca arg 25} P 5.052 12.23 .462
{cb arg 25} 4.666 12.607 1.898
{cg arg 25} 4.742 11.434 2.88
{cd arg 25} 3.944 11.647 4.18
{ne arg 25} 4.79 12.048 5.31
{cz arg 25} 4.757 13.214 5.978
{nh1 arg 25} 5.61 13.455 6.983
{cz arg 25} P 4.757 13.214 5.978
{nh2 arg 25} 3.87 14.156 5.643
{ca lys 28} P 3.73 11.297 -4.703
{cb lys 28} 3.682 10.178 -3.632
{cg lys 28} 2.817 8.988 -4.033
{cd lys 28} 3.2 7.636 -3.433
{ce lys 28} 3.947 7.66 -2.113
{nz lys 28} 4.214 6.285 -1.67
{ca arg 1} P 38.127 14.684 15.695
{cb arg 1} 39.474 14.7 15.01
{cg arg 1} 40.613 14.837 16.017
{cd arg 1} 40.603 16.12 16.852
{ne arg 1} 40.475 17.405 16.157
{cz arg 1} 40.623 17.72 14.874
{nh1 arg 1} 40.45 18.986 14.516
{cz arg 1} P 40.623 17.72 14.874
{nh2 arg 1} 40.95 16.805 13.947
{ca glu 6} P 32.395 20.303 12.225
{cb glu 6} 33.53 21.306 12.328
{cg glu 6} 33.764 21.673 13.808
{cd glu 6} 34.96 22.544 14.106
{oe1 glu 6} 35.762 22.895 13.268
{cd glu 6} P 34.96 22.544 14.106
{oe2 glu 6} 35.036 22.89 15.368
{ca lys 8} P 29.714 16.773 9.066
{cb lys 8} 29.793 15.666 10.055
{cg lys 8} 28.684 14.726 9.758
{cd lys 8} 29.11 13.708 8.745
{ce lys 8} 27.965 12.797 8.393
{nz lys 8} 27.772 12.684 6.944
{ca leu 13} P 22.953 21.628 7.228
{cb leu 13} 23.716 22.736 7.946
{cg leu 13} 22.928 23.994 7.907
{cd1 leu 13} 21.708 23.797 8.794
{cg leu 13} P 22.928 23.994 7.907
{cd2 leu 13} 23.81 25.215 8.21
{ca lys 15} P 21.673 18.905 2.595
{cb lys 15} 21.79 17.483 3.008
{cg lys 15} 23.038 16.816 2.568
{cd lys 15} 22.765 15.344 2.585
{ce lys 15} 23.758 14.586 3.423
{nz lys 15} 23.747 13.146 3.13
{ca glu 20} P 13.734 22.442 2.62
{cb glu 20} 14.172 23.41 3.73
{cg glu 20} 14.123 22.844 5.175
{cd glu 20} 14.912 23.638 6.227
{oe1 glu 20} 14.944 24.867 6.317
{cd glu 20} P 14.912 23.638 6.227
{oe2 glu 20} 15.543 22.856 7.072
{ca glu 22} P 12.464 21.235 -2.495
{cb glu 22} 12.98 19.798 -2.45
{cg glu 22} 14.167 19.59 -3.386
{cd glu 22} 13.732 19.647 -4.82
{oe1 glu 22} 13.137 18.715 -5.31
{cd glu 22} P 13.732 19.647 -4.82
{oe2 glu 22} 14.023 20.778 -5.467
{ca lys 27} P 4.334 23.132 -1.617
{cb lys 27} 4.805 23.734 -.3
{cg lys 27} 4.842 22.606 .72
{cd lys 27} 4.832 22.957 2.193
{ce lys 27} 4.752 21.638 2.957
{nz lys 27} 4.815 21.72 4.424
{ca leu 29} P 2.917 22.842 -6.655
{cb leu 29} 3.6 21.478 -6.794
{cg leu 29} 4.85 21.47 -7.638
{cd1 leu 29} 4.88 20.21 -8.47
{cg leu 29} P 4.85 21.47 -7.638
{cd2 leu 29} 4.944 22.693 -8.533
{ca arg 1} P 32.954 20.476 22.253
{cb arg 1} 33.484 20.438 23.69
{cg arg 1} 32.988 19.262 24.48
{cd arg 1} 31.497 19.365 24.41
{ne arg 1} 30.73 18.684 25.417
{cz arg 1} 29.9 19.248 26.292
{nh1 arg 1} 29.726 20.57 26.46
{cz arg 1} P 29.9 19.248 26.292
{nh2 arg 1} 29.24 18.42 27.073
{ca glu 6} P 28.047 14.698 17.988
{cb glu 6} 28.596 13.52 18.826
{cg glu 6} 30.132 13.372 18.716
{cd glu 6} 30.73 12.584 19.86
{oe1 glu 6} 30.067 12.113 20.764
{cd glu 6} P 30.73 12.584 19.86
{oe2 glu 6} 32.016 12.382 19.742
{ca lys 8} P 24.16 18.326 16.627
{cb lys 8} 25.208 19.411 16.453
{cg lys 8} 24.875 20.453 15.448
{cd lys 8} 23.607 21.098 15.875
{ce lys 8} 23.57 22.52 15.433
{nz lys 8} 22.612 23.272 16.228
{ca leu 13} P 20.506 13.546 10.735
{cb leu 13} 21.36 12.378 11.302
{cg leu 13} 21.198 11.12 10.452
{cd1 leu 13} 21.59 11.418 8.997
{cg leu 13} P 21.198 11.12 10.452
{cd2 leu 13} 21.886 9.878 11.052
{ca lys 15} P 15.768 16.242 10.722
{cb lys 15} 16.472 17.52 10.958
{cg lys 15} 15.594 18.56 10.372
{cd lys 15} 15.965 19.924 10.847
{ce lys 15} 15.38 20.966 9.92
{nz lys 15} 14.91 22.17 10.62
{ca glu 20} P 13.32 13.222 2.974
{cb glu 20} 14.365 12.12 3.203
{cg glu 20} 15.78 12.685 3.263
{cd glu 20} 16.387 12.59 1.915
{oe1 glu 20} 15.69 12.585 .902
{cd glu 20} P 16.387 12.59 1.915
{oe2 glu 20} 17.703 12.458 1.957
{ca glu 22} P 8.192 14.685 3.595
{cb glu 22} 8.55 15.985 4.356
{cg glu 22} 7.565 17.187 4.184
{cd glu 22} 8.016 18.4 5.
{oe1 glu 22} 8.942 18.366 5.777
{cd glu 22} P 8.016 18.41 5.
{oe2 glu 22} 7.337 19.528 4.817
{ca lys 27} P 6.086 14.333 -4.63
{cb lys 27} 7.445 13.675 -4.847
{cg lys 27} 8.562 14.638 -5.223
{cd lys 27} 9.948 14. -5.2
{ce lys 27} 10.793 14.34 -6.426
{nz lys 27} 11.577 15.578 -6.283
{ca leu 29} P .8 13.434 -3.964
{cb leu 29} .692 14.17 -2.63
{cg leu 29} -.5 15.08 -2.588
{cd1 leu 29} -1.573 14.266 -1.91
{cg leu 29} P -.5 15.081 -2.588
{cd2 leu 29} -.185 16.36 -1.8
@kinemage 2
@caption
Calpha trace of the coiled coil protein, tropomyosin.
@onewidth 
@matrix 
.085219 -.377187 .922208 -.290544 .875941 .385112 -.953059 -.300761 -.034943 
@group {TROPOMY.} 
@subgroup {Chain 1}
@vectorlist {Alpha-C's} color=sky
{asp a 41} P -33.737 79.024 -304.253
{glu a 42} -35.31 78.3 -300.89
{leu a 43} -32.17 79.35 -299.084
{val a 44} -32.04 82.615 -300.984
{ser a 45} -35.663 83.34 -300.145
{leu a 46} -35.064 82.546 -296.5
{gln a 47} -32.063 84.83 -296.384
{lys a 48} -34.056 87.684 -297.88
{lys a 49} -36.853 87.14 -295.383
{leu a 50} -34.405 87.075 -292.51
{lys a 51} -32.847 90.333 -293.62
{gly a 52} -36.256 91.984 -293.84
{thr a 53} -37.193 90.69 -290.413
{glu a 54} -33.98 91.997 -288.924
{asp a 55} -34.59 95.44 -290.38
{glu a 56} -38.134 95.467 -289.03
{leu a 57} -36.94 94.39 -285.613
{asp a 58} -34.374 97.16 -285.516
{lys a 59} -37.004 99.74 -286.43
{tyr a 60} -39.353 98.396 -283.787
{ser a 61} -36.647 98.53 -281.16
{glu a 62} -35.887 102.15 -281.99
{ala a 63} -39.57 103.043 -281.787
{leu a 64} -39.91 101.26 -278.48
{lys a 65} -36.956 103.12 -277.043
{asp a 66} -38.424 106.447 -278.12
{ala a 67} -41.78 105.526 -276.64
{gln a 68} -40.176 104.52 -273.38
{glu a 69} -38.34 107.817 -273.15
{lys a 70} -41.543 109.72 -273.83
{leu a 71} -43.39 107.693 -271.24
{glu a 72} -40.73 108.373 -268.65
{leu a 73} -40.9 112.09 -269.34
{ala a 74} -44.67 112.02 -269.105
{glu a 75} -44.53 110.127 -265.846
{lys a 76} -42.12 112.64 -264.386
{lys a 77} -44.35 115.49 -265.47
{ala a 78} -47.377 113.77 -264.02
{thr a 79} -45.61 113.216 -260.736
{asp a 80} -44.62 116.85 -260.576
{ala a 81} -48.154 117.904 -261.38
{glu a 82} -49.533 115.594 -258.737
{ala a 83} -47.16 116.98 -256.16
{asp a 84} -48.127 120.5 -257.093
{val a 85} -51.786 119.607 -256.923
{ala a 86} -51.337 118.03 -253.53
{ser a 87} -49.54 121.085 -252.276
{leu a 88} -52.25 123.304 -253.666
{asn a 89} -54.92 121.17 -252.084
{arg a 90} -53.17 121.3 -248.757
{arg a 91} -52.846 125.035 -249.03
{ile a 92} -56.483 125.346 -249.97
{gln a 93} -57.51 123.19 -247.054
{leu a 94} -55.417 125.25 -244.716
{val a 95} -56.89 128.42 -246.124
{glu a 96} -60.38 127.046 -245.774
{glu a 97} -59.72 126.06 -242.205
{glu a 98} -58.32 129.47 -241.48
{leu a 99} -61.294 131.11 -243.11
{asp a 100} -63.673 128.975 -241.115
{arg a 101} -61.854 129.82 -237.94
{ala a 102} -61.915 133.48 -238.82
{gln a 103} -65.604 133.32 -239.58
{glu a 104} -66.286 131.607 -236.296
{arg a 105} -64.233 134.18 -234.476
{leu a 106} -66.04 136.977 -236.245
{ala a 107} -69.39 135.49 -235.367
{thr a 108} -68.36 135.14 -231.764
{ala a 109} -67.107 138.694 -231.717
{leu a 110} -70.33 139.944 -233.224
{gln a 111} -72.354 138.07 -230.65
{lys a 112} -70.204 139.45 -227.876
{leu a 113} -70.55 142.95 -229.235
{glu a 114} -74.306 142.613 -229.41
{glu a 115} -74.427 141.36 -225.85
{ala a 116} -72.216 144.195 -224.7
{glu a 117} -74.394 146.73 -226.45
{lys a 118} -77.5 145.323 -224.813
{ala a 119} -75.827 145.397 -221.423
{ala a 120} -74.71 148.963 -221.95
{asp a 121} -78.21 150.03 -222.884
{glu a 122} -79.63 148.37 -219.783
{ser a 123} -77.004 150.01 -217.61
{glu a 124} -77.755 153.404 -219.08
{arg a 125} -81.46 152.99 -218.39
{gly a 126} -80.754 151.936 -214.815
{met a 127} -78.46 154.887 -214.294
{lys a 128} -81.085 157.294 -215.555
{val a 129} -83.68 155.83 -213.2
{ile a 130} -81.283 156.045 -210.28
{glu a 131} -80.5 159.657 -211.045
{ser a 132} -84.19 160.55 -211.107
{arg a 133} -84.756 158.8 -207.783
{ala a 134} -81.807 160.575 -206.23
{gln a 135} -83.1 163.946 -207.35
{lys a 136} -86.52 163.238 -205.84
{asp a 137} -84.95 162.12 -202.58
{glu a 138} -82.84 165.243 -202.396
{glu a 139} -85.885 167.455 -202.876
{lys a 140} -87.774 165.604 -200.146
{met a 141} -84.843 165.9 -197.78
{glu a 142} -84.61 169.63 -198.353
{ile a 143} -88.313 170.07 -197.615
{gln a 144} -88.02 168.006 -194.45
{glu a 145} -85.063 170.034 -193.273
{ile a 146} -86.954 173.28 -193.774
{gln a 147} -89.935 171.947 -191.837
{leu a 148} -87.704 170.783 -189.02
{lys a 149} -86.05 174.176 -188.77
{glu a 150} -89.433 175.903 -188.576
{ala a 151} -90.605 173.5 -185.895
{lys a 152} -87.47 174.065 -183.87
{his a 153} -87.926 177.82 -184.02
{ile a 154} -91.536 177.51 -182.9
{ala a 155} -90.554 175.237 -180.05
{glu a 156} -87.91 177.67 -178.88
{asp a 157} -90.397 180.527 -178.91
{ala a 158} -92.91 178.467 -176.977
{asp a 159} -90.317 177.526 -174.404
{arg a 160} -89.35 181.15 -173.906
{lys a 161} -92.98 182.14 -173.48
{tyr a 162} -93.54 179.355 -170.99
{glu a 163} -90.53 180.413 -168.973
{glu a 164} -91.74 183.996 -168.88
{val a 165} -95.19 182.88 -167.81
{ala a 166} -93.75 180.73 -165.067
{arg a 167} -91.675 183.607 -163.765
{lys a 168} -94.694 185.884 -163.776
{leu a 169} -96.76 183.3 -161.963
{val a 170} -94.1 182.88 -159.32
{ile a 171} -93.913 186.62 -158.795
{ile a 172} -97.675 186.84 -158.53
{glu a 173} -97.766 184.036 -156.01
{ser a 174} -95.13 185.727 -153.896
{asp a 175} -97.025 188.995 -154.02
{leu a 176} -100.24 187.27 -153.06
{glu a 177} -98.577 185.59 -150.117
{arg a 178} -97.16 188.89 -148.936
{ala a 179} -100.536 190.55 -149.29
{glu a 180} -102.21 187.79 -147.335
{glu a 181} -99.683 188.114 -144.54
{arg a 182} -100.17 191.86 -144.444
{ala a 183} -103.92 191.46 -144.36
{glu a 184} -103.7 189.02 -141.49
{leu a 185} -101.48 191.39 -139.546
{ser a 186} -103.837 194.263 -140.216
{glu a 187} -106.8 192.232 -139.064
{gly a 188} -105.037 191.317 -135.844
{lys a 189} -104.127 194.94 -135.235
{cys a 190} -107.677 196.033 -135.895
{ala a 191} -109.026 193.49 -133.453
{glu a 192} -106.6 194.65 -130.786
{leu a 193} -107.523 198.26 -131.417
{glu a 194} -111.203 197.47 -131.16
{glu a 195} -110.69 195.71 -127.85
{glu a 196} -108.69 198.64 -126.53
{ile a 197} -111.335 201.077 -127.67
{lys a 198} -114.05 199.1 -125.94
{thr a 199} -112.06 199.045 -122.714
{val a 200} -111.427 202.763 -122.96
{thr a 201} -115.09 203.453 -123.536
{asn a 202} -116.05 201.427 -120.49
{asn a 203} -113.49 203.26 -118.38
{leu a 204} -114.716 206.6 -119.646
{lys a 205} -118.29 205.73 -118.796
{ser a 206} -117.29 204.717 -115.284
{leu a 207} -115.31 207.907 -114.857
{glu a 208} -118.216 209.99 -116.054
{ala a 209} -120.55 208.325 -113.587
{gln a 210} -118.09 208.91 -110.767
{ala a 211} -117.675 212.525 -111.773
{glu a 212} -121.413 213.05 -111.81
{lys a 213} -121.736 211.554 -108.35
{tyr a 214} -118.906 213.714 -107.81
{ser a 215} -120.474 216.807 -108.563
{gln a 216} -123.78 216.04 -106.904
{lys a 217} -122.06 215.51 -103.58
{glu a 218} -120.136 218.733 -103.974
{asp a 219} -123.297 220.64 -104.75
{lys a 220} -124.99 219.225 -101.68
{tyr a 221} -121.995 220.094 -99.553
{glu a 222} -121.924 223.606 -100.923
{glu a 223} -125.59 224.076 -100.16
{glu a 224} -125.08 222.8 -96.64
{ile a 225} -122.105 225.074 -96.174
{lys a 226} -124.07 228.055 -97.39
{val a 227} -126.884 227.26 -95.01
{leu a 228} -124.44 226.87 -92.164
{ser a 229} -122.777 230.143 -93.023
{asp a 230} -126.103 231.914 -93.087
{lys a 231} -127.02 230.425 -89.745
{leu a 232} -123.675 231.416 -88.31
{lys a 233} -124.07 234.944 -89.58
{glu a 234} -127.513 235.173 -88.067
{ala a 235} -126.22 233.825 -84.79
{glu a 236} -123.36 236.28 -84.81
{thr a 237} -125.714 239.146 -85.47
{arg a 238} -127.96 238.006 -82.67
{ala a 239} -125.01 237.675 -80.345
{glu a 240} -123.806 241.135 -81.235
@subgroup {Chain 2} 
@vectorlist {Alpha-C's} color=sea
{asp b 41} P -28.15 73.03 -294.53
{glu b 42} -27.716 76.69 -295.43
{leu b 43} -31.425 77.076 -296.027
{val b 44} -32.237 75.54 -292.673
{ser b 45} -29.85 77.9 -290.914
{leu b 46} -31.335 80.873 -292.72
{gln b 47} -34.837 79.81 -291.8
{lys b 48} -33.89 79.49 -288.156
{lys b 49} -32.31 82.925 -288.19
{leu b 50} -35.356 84.4 -289.86
{lys b 51} -37.64 82.843 -287.29
{gly b 52} -35.545 84.22 -284.47
{thr b 53} -35.547 87.647 -286.054
{glu b 54} -39.29 87.54 -286.506
{asp b 55} -39.79 86.574 -282.894
{glu b 56} -37.53 89.376 -281.77
{leu b 57} -39.36 91.833 -283.974
{asp b 58} -42.693 90.72 -282.607
{lys b 59} -41.45 91.095 -279.075
{tyr b 60} -40.09 94.524 -279.856
{ser b 61} -43.354 95.555 -281.436
{glu b 62} -45.283 94.354 -278.43
{ala b 63} -42.953 96.21 -276.133
{leu b 64} -43.276 99.34 -278.213
{lys b 65} -47.04 99.09 -278.167
{asp b 66} -47.02 98.63 -274.435
{ala b 67} -44.704 101.574 -274.03
{gln b 68} -46.894 103.713 -276.23
{glu b 69} -49.953 102.757 -274.25
{lys b 70} -48.16 103.516 -271.03
{leu b 71} -47.007 106.845 -272.373
{glu b 72} -50.51 107.726 -273.45
{leu b 73} -51.85 106.78 -270.064
{ala b 74} -49.16 108.807 -268.376
{glu b 75} -49.913 111.79 -270.553
{lys b 76} -53.59 111.54 -269.76
{lys b 77} -52.824 111.26 -266.08
{ala b 78} -50.52 114.236 -266.255
{thr b 79} -53.154 116.294 -268.
{asp b 80} -55.71 115.33 -265.4
{ala b 81} -53.29 116.16 -262.63
{glu b 82} -52.54 119.52 -264.164
{ala b 83} -56.224 120.315 -264.43
{asp b 84} -56.76 119.266 -260.844
{val b 85} -53.84 121.367 -259.707
{ala b 86} -55.186 124.39 -261.52
{ser b 87} -58.59 123.906 -259.94
{leu b 88} -57.02 123.495 -256.53
{asn b 89} -54.99 126.64 -256.98
{arg b 90} -58.08 128.604 -257.933
{arg b 91} -59.943 127.24 -254.934
{ile b 92} -57.06 128.09 -252.65
{gln b 93} -56.947 131.637 -253.943
{leu b 94} -60.673 132.04 -253.37
{val b 95} -60.36 130.61 -249.886
{glu b 96} -57.52 132.953 -249.067
{glu b 97} -59.54 135.947 -250.197
{glu b 98} -62.5 134.817 -248.115
{leu b 99} -60.29 134.29 -245.104
{asp b 100} -58.834 137.756 -245.43
{arg b 101} -62.304 139.28 -245.61
{ala b 102} -63.413 137.286 -242.594
{gln b 103} -60.383 138.376 -240.63
{glu b 104} -61.07 142.017 -241.404
{arg b 105} -64.69 141.633 -240.34
{leu b 106} -63.65 139.903 -237.147
{ala b 107} -61.225 142.67 -236.31
{thr b 108} -63.91 145.297 -236.83
{ala b 109} -66.336 143.343 -234.68
{leu b 110} -63.77 142.987 -231.936
{gln b 111} -63.114 146.71 -231.93
{lys b 112} -66.83 147.44 -231.736
{leu b 113} -67.247 144.965 -228.916
{glu b 114} -64.415 146.535 -226.977
{glu b 115} -65.94 149.98 -227.37
{ala b 116} -69.317 148.684 -226.263
{glu b 117} -67.793 147.01 -223.245
{lys b 118} -66.044 150.204 -222.234
{ala b 119} -69.267 152.16 -222.58
{ala b 120} -71.14 149.584 -220.555
{asp b 121} -68.553 149.707 -217.81
{glu b 122} -68.764 153.48 -217.666
{ser b 123} -72.54 153.33 -217.53
{glu b 124} -72.43 150.77 -214.76
{arg b 125} -70.067 152.91 -212.74
{gly b 126} -72.297 155.93 -213.197
{met b 127} -75.34 153.94 -212.186
{lys b 128} -73.61 152.685 -209.074
{val b 129} -72.61 156.2 -208.114
{ile b 130} -76.137 157.43 -208.673
{glu b 131} -77.55 154.63 -206.58
{ser b 132} -75.175 155.416 -203.75
{arg b 133} -76.1 159.077 -203.9
{ala b 134} -79.78 158.236 -203.89
{gln b 135} -79.36 155.99 -200.884
{lys b 136} -77.5 158.695 -199.015
{asp b 137} -80.17 161.227 -199.874
{glu b 138} -82.9 158.893 -198.71
{glu b 139} -81.13 158.32 -195.416
{lys b 140} -80.697 162.043 -194.916
{met b 141} -84.34 162.664 -195.694
{glu b 142} -85.424 160.035 -193.21
{ile b 143} -83.237 161.554 -190.52
{gln b 144} -84.6 165.003 -191.25
{glu b 145} -88.154 163.75 -191.06
{ile b 146} -87.5 162.12 -187.71
{gln b 147} -85.93 165.294 -186.377
{leu b 148} -88.85 167.36 -187.59
{lys b 149} -91.32 165.05 -185.9
{glu b 150} -89.413 165.26 -182.632
{ala b 151} -89.276 169.03 -182.853
{lys b 152} -92.99 169.236 -183.495
{his b 153} -93.73 167.077 -180.472
{ile b 154} -91.48 169.21 -178.29
{ala b 155} -93.127 172.38 -179.525
{glu b 156} -96.57 171.023 -178.744
{asp b 157} -95.5 170.087 -175.224
{ala b 158} -93.98 173.51 -174.68
{asp b 159} -97.133 175.216 -175.863
{arg b 160} -99.25 173.18 -173.467
{lys b 161} -96.935 174.01 -170.58
{tyr b 162} -97.016 177.687 -171.44
{glu b 163} -100.796 177.704 -171.5
{glu b 164} -100.946 176.043 -168.088
{val b 165} -98.457 178.52 -166.676
{ala b 166} -100.434 181.44 -168.024
{arg b 167} -103.62 180.153 -166.424
{lys b 168} -101.863 179.7 -163.09
{leu b 169} -100.41 183.18 -163.275
{val b 170} -103.81 184.683 -163.957
{ile b 171} -105.31 182.875 -160.973
{ile b 172} -102.47 184.022 -158.75
{glu b 173} -102.895 187.6 -159.874
{ser b 174} -106.6 187.513 -159.09
{asp b 175} -105.915 186.087 -155.642
{leu b 176} -103.29 188.72 -154.975
{glu b 177} -105.663 191.49 -155.96
{arg b 178} -108.34 190.14 -153.637
{ala b 179} -105.85 189.87 -150.8
{glu b 180} -104.673 193.41 -151.364
{glu b 181} -108.223 194.71 -151.264
{arg b 182} -108.9 192.83 -148.05
{ala b 183} -105.715 194.15 -146.51
{glu b 184} -106.62 197.7 -147.43
{leu b 185} -110.05 197.312 -145.88
{ser b 186} -108.54 195.85 -142.737
{glu b 187} -106.05 198.677 -142.502
{gly b 188} -108.8 201.247 -142.84
{lys b 189} -110.825 199.53 -140.153
{cys b 190} -107.82 199.37 -137.88
{ala b 191} -107.123 203.043 -138.376
{glu b 192} -110.71 203.907 -137.57
{leu b 193} -110.6 201.74 -134.48
{glu b 194} -107.377 203.34 -133.36
{glu b 195} -108.83 206.79 -133.805
{glu b 196} -111.906 205.81 -131.85
{ile b 197} -109.78 204.33 -129.11
{lys b 198} -107.694 20