The Newcomb Art Gallery collections are focused on the crafts produced at Newcomb College from the late 19th through the early 20th century with more than 400 examples of pottery, metal work, embroidery and bound books. The largest holdings are in the area of Newcomb Pottery, including a number of rare exhibition-only and early experimental pieces. Additionally, the Gallery holds paintings, drawings, sculptures and photographs by past and present faculty and students of the Newcomb Art Department. Notable, as well, are the two Tiffany window triptychs that once adorned the Newcomb Chapel and are now installed in Woodward Way flanking the entrance to the Gallery.

The Newcomb Art Gallery is also guardian of the Tulane University Art Collections, which include a diverse array of works acquired since the school's founding. Some of these objects are on display in Tulane's administrative offices and public spaces.