|
|
The Tulane Transgenic Mouse Facility |
In Vitro Fertilization
In vitro fertilization (IVF) can facilitate the recovery and expansion of mouse lines as sperm from one mouse can potentially produce hundreds of offspring using IVF. IVF is used to recover frozen sperm and oocytes and be used to expand new strains brought in from other universities. This technique also has the potential to rescue valuable strains that fail to breed.
Investigators must supply the following items
- Completed Request for Service form
- At least 2 proven breeder males,
- If homozygous offspring are essential, at least 16 females, 3-4 weeks old, are required.
- Current protocol number for the pups
- IT form for facility charges (Tulane only)
General timeline
- Mice arrive
- IVF set up, eggs transferred to foster mother (day 5)
- Foster mice give birth (investigator notified) (day 26)
- Tail tips given to investigator when pups are 15-16 days old (day 42)
- Pups are weaned at 21 days old (47 days)
Special notes
The arrival of mice from any non-approved vendor must be coordinated with the director of the Uptown Vivarium, Kimberley Scamardo, phone number (504) 865-6716.
If the investigator wishes to rederive mice via IVF, additional charges will apply.
IVF with live birth and cryopreservation of embryos and can be combined for an additional charge. In addition, more females are required for this procedure.
If the foster females fail to deliver live pups, the process will be repeated at no additional cost (other than the cost of more mice, if needed).
If the foster females fail to deliver pups of the proper genotype, the process will be repeated at no additional cost (other than the cost of more mice) AFTER the investigator has done appropriate genetic testing on the males to confirm their genotype.
The current charge for Tulane investigators for embryo cryopreservation is $1000 and $2500 for outside investigators.
The Tulane Transgenic Mouse Facility wishes to acknowledge the Tulane Research Enhancement Fund for awarding funds for the purchase of equipment for IVF and cryopreservation.
Last updated May 2008