SEQEUNCES FOR HOMEWORK #10


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Plasmid containing insert DNA:


  | XhoI || SalI |    | ClaI||NsiI|                   | SalI|           |EcoRI || PstI || SmaI || BamHI|| SpeI || XbaI |  
CCC TCG AGG TCG ACG GTA TCG ATG CAT .....insert.......GTC GAC CAC CCA TCG AAT TCC TGC AGC CCG GGG GAT CCA CTA GTT CTA GAG 
GGG AGC TCC AGC TGC CAT AGC TAC GTA ..................CAG CTG GTG GGT AGC TTA AGG ACG TCG GGC CCC CTA GGT GAT CAA GAT CTC 
Pro Ser Arg Ser Thr Val Ser Met His ..................Val Asp His Pro Ser Asn Ser Cys Ser Pro Gly Asp Pro Leu Val Leu Glu 



pGEX-5X-3:

                           |BamH1 |     |EcoR1 ||SmaI |SalI | XhoI |  NotI  |
...GST... ATC GAA GGT CGT GGG ATC CCC AGG AAT TCC CGG GTC GAC TCG AGC GGC CGC ATC GTG ACT GAC TGA
......... TAG CTT CCA GCA CCC TAG GGG TCC TTA AGG GCC CAG CTG AGC TCG CCG GCGT AG CAC TGA CTG ACT
......... Ile Glu Gly Arg Gly Ile Pro Arg Asn Ser Arg Val Asp Ser Ser Gly Arg Ile Val Thr Asp Stop
          |  Factor Xa  |